Ultrastructural Analysis of Spores and Parasporal Crystals Formed by Bacillus sphaericus 2297
نویسندگان
چکیده
منابع مشابه
Ultrastructural analysis during germination and outgrowth of Bacillus subtilis spores.
Electron microscopy of thin sections of dormant and germinating spores of Bacillus subtilis 168 revealed a progressive change in the structure of the cortex, outer spore coat, and inner spore coat. The initial changes were observed in the cortex region, which showed a loose fibrous network within 10 min of germination, and in the outer spore coat, which began to be sloughed off. The permeabilit...
متن کاملRelationship between cortex content and properties of Bacillus sphaericus spores.
The muramic lactam content of spores of Bacillus sphaericus mutants defective in meso-diaminopimelic acid synthesis increases almost linearly with an increase of meso-diaminopimelic acid concentration in the medium. Since muramic lactam content is a measure of cortex content, the amount of cortex in spores of the mutants can be easily varied by changing the meso-diaminopimelic acid concentrati...
متن کاملSeparation of spores and parasporal crystals of Bacillus thuringiensis in gradients of certain x-ray contrasting agents.
Spores and parasporal crystals of Bacillus thuringiensis can be separated at moderate centrifugation speeds (10,000 to 12,000 rpm) in gradients of Renografin or sodium diatrizoate.
متن کاملBacillus sphaericus strain 2297: nucleotide sequence of 41.9 kDa toxin gene.
We previously reported the nucleotide and deduced amino acid sequences of B. sphaericus 1593 and 2362 41.9kDa larvicldal toxin genes (1 4 2). Both these strains belonged to the same H-flagellar antigen group (H 5a, 5b) and possessed Identical.coding sequences in an open reading frame of 1110 nucleotides. Using restriction fragment probes derived from these sequences we have identified and clone...
متن کاملMorphological and Chemical Studies of the Spores and Parasporal Bodies of Bacillus laterosporus
Spores of Bacillus laterosporus were studied to determine the chemical and morphological nature of their basophilic canoe-shaped parasporal bodies. An unusually high phosphorus content of these spores compared to other Bacillus species appeared to be associated with the parasporal body. Preparations of these "canoes" still attached to the spore coats were indeed high in phosphorus, but also in ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1982
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.44.6.1449-1455.1982